Composite

Part:BBa_K1465208:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-02)


Carbonic anhydrase (csoS3) and shell-proteins (csoS14) of the carboxysome of H. neapolitanus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 166
    Illegal AgeI site found at 2277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the shell-proteins (BBa_K1465206) into the pSB1C3 backbone of the carbonic anhydrase (BBa_K1465205).

Source

BBa_K1465206: shell proteins csoS4AB and csoS1CAB from Halothiobacillus neapolitanus.
BBa_K1465205: carbonic anhydrase csoS3 (can) from Halothiobacillus neapolitanus.

References